"Dead Man's Blood"
With the introduction of Daniel Elkins, a hunter who showed John the ropes, comes the introduction of the Colt, the gun that (supposedly) can kill anything. This gun, created along with 13 bullets by Sam Colt in 1835 during Halley's comet, will kill the demon who killed Mary, and John wants that gun!
John and Sam have a touching scene during which Papa W. explains why he didn't want him going away to school"he feared Sam being alone, away from the protection of the family. He did start a college fund for both him and Dean, but spent it on ammo. He and Sam have a big laugh over that, and it seems to bring the two of them closer. Meanwhile, Dean's been dispatched to a funeral home to pick up a jar of dead man's blood, which renders vampires unconscious if injected into them. There is also a lot of head-butting between John and Sam, who resents being told what to do without being given reasons and details. Unlike Dean, who's fine having John give them orders to blindly follow, Sam wants to know why they are doing what they're doing.
Vampires! Luther insists, "We have just as much a right to live as you do." Do they, given that they're murdering people, turning them into vamps? John thought them extinct, Dean and Sam thought there was no such thing. These badass vamps drink hard Jack; capture their victims by stretching out in the street and playing injured victim; kill the husband they don't like by feasting on his blood in front of his wife; have sex in front of the wife, then feed her vampire blood; kill hunter Daniel Elkins and steal the Colt from him; party hearty in subdued sunlight; sleep in hammocks; and mate for life!
John captures Kate, Luther's mate, by striking her with an arrow coated in dead man's blood. They capture another from the nest, and John beheads him. John tasks his sons to wipe out the rest of the nest while he exchanges Kate for the Colt. Then he wants them gone, and he will take the Colt and go after the demon alone. Losing Mary nearly killed him; he will not risk his children, too! Sam and Dean have a problem with this plan and tell him so. John, not used to mutiny from Dean, sends them on their way. John's plan goes awry, however. When the time comes, Kate, free of both the influence of the blood and her bonds, attacks John. The Colt spins away on the street. Dean and Sam appear and subdue two of the vamps with arrows coated in DMB, but Sam finds himself in Luther's chokehold and Dean is forced to lay down his sword. John shoots a bullet from the Colt into Luther's forehead. It kills him slowly, with great special affects.
John reminds his sons that they ignored his direct order. "Saved your ass," Dean reminds him. Much as he hates to admit it, Dean and Sam are all he's got"his family"and they'll go after the demon together. "Yes sir," says Sam. Both brothers are smiling.