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6) How many themes that run throughout Supernatural - even to this day - do you see introduced in the pilot episode?
Elle - So many themes are established. Family and brotherhood being the most fundamental. The influence of Mary, and John. The warrior elements of what Sam and Dean do. And, finally, why they do it - in this case, for example they were on a mission to find their father and could have kept going, but stayed to finish the hunt even after they knew he was gone. It's the foundation of "saving people, hunting things" from the beginning that still runs through. The mission started with a revenge thing and at times takes that turn again, but always one pulls the other back to look at the bigger picture again.
Elle2 - Family, revenge, redemption, destiny, lies: they’re all there. We see the lives of these two young men on the fringes of society, which is where they still exist today; we see them endlessly hunting something, because as Dean said in Shadow (and as exemplified here) there will always be something to hunt. There are three hunts either occurring or spoken of in the Pilot: The Woman in White, John gets a lead on the demon, and upon leaving, leaves Dean coordinates to the next hunt: Blackwater Ridge, Colorado (Wendigo). The same continues today, there is always something to hunt, and the past does come back to haunt you: werewolves, shapeshifters, and vampires remain in Season 10, when they were supposed to be resting, and the demon came for Jessica at the end of the Pilot.
Percysowner - Sam's desire for a normal life, Dean's commitment to hunting. Dean's devotion to John and his refusal to see John in a negative way still comes up, for example in Bad Boys he defends John for leaving him at the boy's home, in spite of the fact that he could have been angry.
Metamorphic Rocks - There are so many props, trademark behaviors, and show themes that have run through 9.5 seasons introduced in this episode: Classic rock, pop culture references, fake IDs, impersonating law enforcement, the Impala, the victim's blood spatter, and John's journal are all there. The dynamics of the brothers' relationship are already hinted at with "Sammy," "Jerk/Bitch," "the driver picks the music, shotgun shuts his cake hole," lines, as well as more serious tensions, including Sam's rejection of the hunting life, Dean's unyielding devotion to John, and Dean's tendency to see Sam's desire for normal as abandoning Dean.
The pilot did an exceptional job of laying out mythology that would be crucial to the show's canon for all of its seasons (so far), including the ramifications of Mary's death, John's obsessive quest, the brothers strange and dangerous childhood, evil forces targeting Sam for reasons unknown, and of course, Dean's protectiveness over Sam. The pilot was packed full of all these things, and more.